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α-MEM 培養(yǎng)基
MEM培養(yǎng)基是一種添加了營養(yǎng)物的極限必需培養(yǎng)基，也稱為最低必需培養(yǎng)基、最小基本培養(yǎng)基或低限量Eagle培養(yǎng)基。它僅包含12種必需氨基酸、谷氨酰胺和8種維生素。MEM是一種最基礎(chǔ)、最常用的培養(yǎng)基，是由Harry Eagle在Eagle基本培養(yǎng)基（BEM）的基礎(chǔ)上發(fā)展而來。在添加血清后，MEM可用于培養(yǎng)多種單層生長的細胞，如成纖維細胞。配方修改后也可用于其他類型的細胞培養(yǎng)。α-MEM是在MEM的基礎(chǔ)上添加了NEAA（非必須氨基酸）、丙酮酸鈉、硫酸鋅、VB12、生物素、抗壞血酸等成分的培養(yǎng)基，廣泛應(yīng)用于各種哺乳動物懸浮和貼壁細胞的培養(yǎng)。7種NEAA包括L-丙氨酸、L-谷氨酸、L-天門冬酰胺、L-天門冬氨酸、L-脯氨酸、L-絲氨酸和甘氨酸，能降低細胞培養(yǎng)時細胞自身生產(chǎn)非必須氨基酸的副作用，有效促進細胞增殖代謝。
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α-MEM培養(yǎng)基；含核苷
500mL/瓶
209041

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